Journal: EXCLI Journal

Article Title: The inflammatory cytokine TNF contributes with RAC3-induced malignant transformation

doi: 10.17179/excli2018-1759

Figure Lengend Snippet: A. Inmunofluorescence analysis of β-Catenin in HEK293 (upper panels) and HeLa (bottom panels) stimulated during 24 h with: vehicle (Basal), TNF 20 ng/ml (TNF), sulfasalazine 250 μM (SZ) and TNF plus sulfasalazine (TNF+SZ). B and C-Reporter assays for TCF/β-Catenin-Luc. The bar diagram shows the average relative light units (RLU) of HEK293 (B) and HeLa (C) stimulated with vehicle (Basal), TNF 20 ng/ml, sulfasalazine 250 μM (SZ), TNF plus sulfasalazine (TNF+SZ) and normalized respect to the β-galactosidase activity at each case. a: p < 0.05 respect to basal RAC3, b: p < 0.05 respect to TNF stimulated RAC3, c: p < 0.05 respect to basal SC, d: p < 0.05 respect to TNF stimulated SC.

Article Snippet: The assays for luciferase and β-galactosidase activity were performed after 48 h of treatment using the appropriate substrates following the manufacturer's protocols (Promega Corp. USA).

Techniques: Activity Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Long-Term Administration of Triterpenoids From Ganoderma lucidum Mitigates Age-Associated Brain Physiological Decline via Regulating Sphingolipid Metabolism and Enhancing Autophagy in Mice

doi: 10.3389/fnagi.2021.628860

Figure Lengend Snippet: Improvement of multiple organ aging after long-term administration of TGL. TGL shows an improvement in brown adipose, liver, and kidney. (A) HE staining of brown adipose tissue; (B) Apoptosis rate of Liver using TUNEL staining; (C) TGL shows an improvement on aging in kidney using β-galactosidase staining. Data are presented as the means ± SD of more than six independent experiments. ** p < 0.01.

Article Snippet: Detection kit for β-galactosidase (β-Gal) activity , , Solarbio , BC2580 , http://www.solarbio.com/.

Techniques: Staining, TUNEL Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Long-Term Administration of Triterpenoids From Ganoderma lucidum Mitigates Age-Associated Brain Physiological Decline via Regulating Sphingolipid Metabolism and Enhancing Autophagy in Mice

doi: 10.3389/fnagi.2021.628860

Figure Lengend Snippet:

Article Snippet: Detection kit for β-galactosidase (β-Gal) activity , , Solarbio , BC2580 , http://www.solarbio.com/.

Techniques: Recombinant, Activity Assay, TUNEL Assay, Staining, RNA Sequencing Assay

Journal: PLoS Pathogens

Article Title: KSHV MicroRNAs Mediate Cellular Transformation and Tumorigenesis by Redundantly Targeting Cell Growth and Survival Pathways

doi: 10.1371/journal.ppat.1003857

Figure Lengend Snippet: (A) IκBα expression in MM cells infected by Mut virus complemented by individual KSHV pre-miRs (MutKi) or vector control (MutVt). Cell grown to confluency were examined for IκBα protein expression by Western-blotting. (B) NF-κB reporter activity in MM cells infected by Mut virus complemented by individual KSHV pre-miRs or vector control. Cells were transfected with a NF-κB reporter construct or it's a mutant reporter together with a β-galatosidase expression construct for 48 h and measured for relative luciferase activities. (C) Deletion of either the miR cluster or vFLIP from the KSHV genome is sufficient to abolish KSHV activation of the NF-κB pathway. Uninfected cells or cells infected by WT and mutants of miRNA cluster (Mut) or vFLIP (ΔvFLIP) were transfected with either NF-κB WT luciferase reporter or NF-κB mutant luciferase reporter together with a β-galactosidase construct for 48 h. Cells were collected and measured for relative luciferase activities. Statistical analyses were performed by comparing other cells with Mut cells (B) or WT cells (C).

Article Snippet: At the indicated time, cells were then lysed, and the luciferase and β-gal activities were measured using luciferase and β-galactosidase kits (Promega).

Techniques: Expressing, Infection, Plasmid Preparation, Western Blot, Activity Assay, Transfection, Construct, Mutagenesis, Luciferase, Activation Assay